X-ray data collection

X-ray data collection within Instruct includes the techniques of x-ray crystallography, small angle scattering and x-ray imaging on synchrotron sources. X-ray crystallography is also available on in house x-ray sources.

X-ray crystallography allows the determination of the three-dimensional shape of proteins at the atomic level and can define interaction surfaces, conserved structural regions and show protein modifications such as glycosylation and methylation. The first step after crystallisation is X-ray data collection, followed by phase determination, calculation of the electron density, model building and model refinement.